Generation of mouse lines expression the human UBE3A antisense

Dr. Beaudet proposed to prepare a series of humanized mouse lines with human DNA insertions of various sizes from 10kb to over 100kb expressing the human UBE3A antisense transcript. All lines will be characterized as to expression of relevant transcripts and for the ability to demonstrate knock-down of the human antisense. One goal of this grant was to make all lines freely and rapidly available to any researchers, academic or corporations working on Angelman syndrome.